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Addgene

pFGC-pcoCas9 Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pFGC-pcoCas9 with the following enzymes: EcoRI-HF, NheI-HF, and BamHI-HF.

Expected Bands
EcoRI-HF/ NheI-HF: 4.5 kb & 8.8 kb
BamHI-HF: 4.9 kb & 8.4 kb

We observe the expected bands for this digest.