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Addgene

pUASp YFP Mtl DN
(Plasmid #52252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASp
  • Backbone size w/o insert (bp) 9939
  • Vector type
    Insect Expression ; Drosophila

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mtl
  • Species
    D. melanogaster (fly)
  • Mutation
    T20N; DN mutation
  • Entrez Gene
    Mtl (a.k.a. Dmel_CG5588, 5588, CG5588, Dmel\CG5588, MTL, Mt1/Mig2-like, Rac, Rac GTPase, Rac-like, dMig-2, mtl)
  • Promoter GAL4
  • Tag / Fusion Protein
    • YFP (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer pUASp-3 (5'-AGGTTTAACCAGGGGATGCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASp YFP Mtl DN was a gift from Matthew Scott (Addgene plasmid # 52252 ; http://n2t.net/addgene:52252 ; RRID:Addgene_52252)
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