pUASp YFP Cdc42 CA
(Plasmid
#52250)
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PurposeDrosophila expression of YFP-Cdc42 constitutively active mutant
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52250 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUASp
- Backbone size w/o insert (bp) 9939
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Vector typeInsect Expression ; Drosophila
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCdc42
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SpeciesD. melanogaster (fly)
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MutationQ61L; CA mutation
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Entrez GeneCdc42 (a.k.a. Dmel_CG12530, CDC-42, CDC42, CG12530, Cdc 42, Cdc-42, Cdc42Dm, D-CDC42, D-Cdc42, D-cdc42, DCDC42, DCdc42, Dcdc42, Dm Cdc42, DmCDC42, Dmcdc42, Dmel\CG12530, P40793, cdc42)
- Promoter GAL4
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Tag
/ Fusion Protein
- YFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer pUASp-3 (5'-AGGTTTAACCAGGGGATGCT-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the ORF contains a SNP at A172T.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUASp YFP Cdc42 CA was a gift from Matthew Scott (Addgene plasmid # 52250 ; http://n2t.net/addgene:52250 ; RRID:Addgene_52250)