pUASp YFP Ras85D CA
(Plasmid #52247)

• Purpose: Drosophila expression of YFP-Ras85D constitutively active mutant
• Depositing Lab: Matthew Scott
• Publication: Matt Scott lab plasmids (unpublished)

Backbone

• Vector backbone: pUASp
• Backbone size w/o insert (bp): 9939
• Vector type: Insect Expression ; Drosophila

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ras85D
• Species: D. melanogaster (fly)
• Mutation: Q61L; CA mutation
• Entrez Gene: Ras85D (a.k.a. Dmel_CG9375, C-ras1, CG9375, D-Ras, D-Ras1, D-ras-1, D-ras1, DRAS1, DRas, DRas85D/Ras, Dm Ras1, Dmel\CG9375, Dmras85D, Dras, Dras1, Dras85D, E(faf), E(sev)3C, EC3-3, EK3-4, P08646, RAS, RAS1, RAS85D, RTK, Ras, Ras 85D, Ras-1, Ras.85D, Ras1, Ras1/RAs85D, Ras185D, Ras85, Ras85DNt, Ras85d, RasI, RasV12, Ras[V12], S35097, Su(tor)3-2, dRAS1, dRas, dRas1, dRas85D, dras1, fs(3)05703, l(3)06677, l(3)s1747, p21[Ras1], ras, ras 1, ras1, ras85B, ras85D, ras[V12])
• Promoter: GAL4
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: pUASp-3 (5'-AGGTTTAACCAGGGGATGCT-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry