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Addgene

pUASp YFP Rac1 WT
(Plasmid #52233)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASp
  • Backbone size w/o insert (bp) 9939
  • Vector type
    Insect Expression ; Drosophila

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rac1
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Rac1 (a.k.a. Dmel_CG2248, 2248, CED-10, CG2248, D-Rac, D-Rac 1, D-Rac1, DRac, DRac1, DRacA, Dm Rac1, DmRAC, DmRAC1, Dmel\CG2248, Dmrac1, Drac, Drac1, Drac1a, DracA, GTPase Rac1, P40792, RAC1, Rac, Rac GTPase, Rac-1, Rac-GTP, Rac3, RacA, dRac, dRac1, drac1, rac, rac-1, rac1)
  • Promoter GAL4
  • Tag / Fusion Protein
    • YFP (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer pUASp-3 (5'-AGGTTTAACCAGGGGATGCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the ORF contains a SNP at K186S.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASp YFP Rac1 WT was a gift from Matthew Scott (Addgene plasmid # 52233 ; http://n2t.net/addgene:52233 ; RRID:Addgene_52233)
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