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Addgene

pMSCV-p19Ink4d-IRES-GFP
(Plasmid #52221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-IRES-GFP
  • Backbone manufacturer
    Reya lab, Addgene Plasmid 20672
  • Backbone size w/o insert (bp) 6573
  • Total vector size (bp) 7200
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ink4d
  • Alt name
    p19
  • Alt name
    cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    500
  • Entrez Gene
    Cdkn2d (a.k.a. INK4d, p19, p19INK4d)
  • Tag / Fusion Protein
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pLXSN5
  • 3′ sequencing primer pCDH-rev (GCATTCCTTTGGCGAGAG)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-p19Ink4d-IRES-GFP was a gift from Martine Roussel (Addgene plasmid # 52221 ; http://n2t.net/addgene:52221 ; RRID:Addgene_52221)

  • For your References section:

    Differential post-transcriptional regulation of two Ink4 proteins, p18 Ink4c and p19 Ink4d. Forget A, Ayrault O, den Besten W, Kuo ML, Sherr CJ, Roussel MF. Cell Cycle. 2008 Dec;7(23):3737-46. Epub 2008 Dec 13. 10.4161/cc.7.23.7187 PubMed 19029828
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