pMSCV-p18Ink4c-IRES-GFP
(Plasmid #52220)

• Purpose: retroviral bicistronic expression of p18Ink4c and GFP
• Depositing Lab: Martine Roussel
• Publication: Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub 2008 Dec 13.

Backbone

• Vector backbone: MSCV-IRES-GFP
• Backbone manufacturer: Reya lab, Addgene Plasmid 20672
• Backbone size w/o insert (bp): 6573
• Total vector size (bp): 7200
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ink4c
• Alt name: cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)
• Alt name: p18
• Species: M. musculus (mouse)
• Insert Size (bp): 500
• Entrez Gene: Cdkn2c (a.k.a. C77269, INK4c, p18, p18-INK4c, p18-INK6, p18INK4c)
• Tag / Fusion Protein:
  • IRES-GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pLXSN5
• 3′ sequencing primer: pCDH-rev (GCATTCCTTTGGCGAGAG)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry