pGEX2T-p18Ink4c
(Plasmid #52218)

• Purpose: bacterial expression of p18Ink4c
• Depositing Lab: Martine Roussel
• Publication: Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub 2008 Dec 13.

Backbone

• Vector backbone: pGEX-2T
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4948
• Total vector size (bp): 5451
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ink4c
• Alt name: cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)
• Alt name: p18
• Species: M. musculus (mouse)
• Insert Size (bp): 500
• Entrez Gene: Cdkn2c (a.k.a. C77269, INK4c, p18, p18-INK4c, p18-INK6, p18INK4c)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry