pIM1773-PRHIII-sfGFP-pNW33N
(Plasmid #52217)

• Purpose: Constitutive sfGFP expression plasmid for use in Geobacillus and E. coli
• Depositing Lab: Ichiro Matsumura
• Publication: Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

Backbone

• Vector backbone: pNW33N
• Backbone manufacturer: Bacillus Genetic Stock Center
• Backbone size w/o insert (bp): 4217
• Total vector size (bp): 5062
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): InvaF'
• Growth instructions: This plasmid is generally slow growing in E. coli. Growth in Geobacillus is generally on par with untransformed strains.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PRHIII-sfGFP
• Species: Synthetic
• Promoter: PRHIII

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: M13-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is designed for use in Geobacillus stearothermophilus NUB3621 (as well as E. coli.) Construct is described in the article linked at the bottom of this page. This plasmid is a companion to pIM1708-PsurP-surT-agaN-pNW33N which was previously submitted. As with pIM1708, despite the fact that your plasmid map displays an ampicillin resistance gene, the correct antibiotic resistance gene is a chloramphenicol acetyltransferase. We do not know if the ampicillin resistance gene is functional.