p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)
(Plasmid #52216)

• Purpose: mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and LCLL mutation
• Depositing Lab: Eric Huang
• Publication: Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2004 Apr 8.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIF1alpha (401delta603) LCLL
• Alt name: HIF1alpha (deltaODD)
• Alt name: hypoxia inducible factor 1, alpha subunit
• Alt name: HIF1alpha
• Species: H. sapiens (human)
• Mutation: aa 401-603 deleted, removes the oxygen-dependent degradation (ODD) domain, and L795V, C800S, L818S, L822V mutations which that disrupt p300/CBP binding
• Entrez Gene: HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH Reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry