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Addgene

pENO1-dTom-NATr
(Plasmid #52208)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52208 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 5800
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dTomato
  • Species
    synthetic construct
  • Insert Size (bp)
    3135
  • Mutation
    codon optimized for Candida species
  • GenBank ID
    AY678268
  • Promoter ENO1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer M13F20
  • 3′ sequencing primer M13Rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid insert was custom synthesized.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENO1-dTom-NATr was a gift from Robert Wheeler (Addgene plasmid # 52208 ; http://n2t.net/addgene:52208 ; RRID:Addgene_52208)

  • For your References section:

    Mucosal candidiasis elicits NF-kappaB activation, proinflammatory gene expression and localized neutrophilia in zebrafish. Gratacap RL, Rawls JF, Wheeler RT. Dis Model Mech. 2013 Sep;6(5):1260-70. doi: 10.1242/dmm.012039. Epub 2013 May 29. 10.1242/dmm.012039 PubMed 23720235
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