pSG5-mERRbeta-short
(Plasmid #52188)

• Purpose: Expresses mouse ERRbeta short form splice variant in mammalian cells
• Depositing Lab: Rebecca Riggins
• Publication: Heckler et al Cell Cycle. 2014 Dec 12:0.

Backbone

• Vector backbone: pSG5
• Backbone manufacturer: Agilent
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 5462
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Estrogen-related receptor beta
• Species: M. musculus (mouse)
• Insert Size (bp): 1362
• Entrez Gene: Esrrb (a.k.a. Err2, Errb, Estrrb, Nr3b2)
• Promoter: SV40

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene #40798
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry