pChlr-8CQ
(Plasmid #52171)

• Purpose: Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A
• Depositing Lab: Huimin Zhao
• Publication: Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Backbone

• Vector backbone: pChlr-pNEB193
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 2280
• Total vector size (bp): 2336
• Modifications to backbone: Replaced the original ampicillin resistance gene Bla with the chloramphenicol resistance gene Cam from pACYC (New England Biolabs) using the Gibson Assembly method.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pumilio homology domain amino acids 284-319
• Alt name: PUM-HD module 8
• Species: H. sapiens (human)
• Insert Size (bp): 36
• Mutation: Changed 12N to 12C in module 8
• GenBank ID: D43951
• Entrez Gene: PUM1 (a.k.a. HSPUM, NEDMSF, PUMH, PUMH1, PUML1, SCA47)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13 For
• 3′ sequencing primer: M13 Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry