pET28-GG-PUF
(Plasmid
#52137)
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Purpose(Empty Backbone) Receiving vector for PUM-HD assembly and expression in E.coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52137 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size (bp) 5930
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Modifications to backboneInsert containing PUM-HD flanking regions 1' and 8', separated by LacZ alpha, cloned using the Gibson Assembly method.
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Vector typeBacterial Expression
- Promoter T7
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Tags
/ Fusion Proteins
- PUM-HD flanking region 1' (N terminal on insert)
- PUM-HD flanking region 8' (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer T7 For
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28-GG-PUF was a gift from Huimin Zhao (Addgene plasmid # 52137 ; http://n2t.net/addgene:52137 ; RRID:Addgene_52137) -
For your References section:
Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. Abil Z, Denard CA, Zhao H. J Biol Eng. 2014 Mar 1;8(1):7. 10.1186/1754-1611-8-7 PubMed 24581042
Included in kit:
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Map uploaded by the depositor.
