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pcdna3 T7 Jip1b(471-707)(C478S and C699T)
(Plasmid #52132)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52132 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JNK interacting protein-1b (JIP-1b)(471-707)(M1+6 )
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    743
  • Mutation
    deleted amino acid 1-470 and changed C478S and C699T
  • GenBank ID
    AF054611.1
  • Entrez Gene
    Mapk8ip1 (a.k.a. IB1, JIP-1, Jip1, Prkm8ip, Skip, mjip-2a)
  • Tag / Fusion Protein
    • T7 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna3 T7 Jip1b(471-707)(C478S and C699T) was a gift from Roger Davis (Addgene plasmid # 52132 ; http://n2t.net/addgene:52132 ; RRID:Addgene_52132)

  • For your References section:

    A mammalian scaffold complex that selectively mediates MAP kinase activation. Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ. Science. 1998 Sep 11;281(5383):1671-4. 10.1126/science.281.5383.1671 PubMed 9733513
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