pcdna3 EE Jip1b
(Plasmid #52127)

• Purpose: a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
• Depositing Lab: Roger Davis
• Publication: Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: JNK interacting protein-1b (JIP-1b)
• Alt name: JIP-1
• Species: M. musculus (mouse)
• Insert Size (bp): 2152
• GenBank ID: AF054611.1
• Entrez Gene: Mapk8ip1 (a.k.a. IB1, JIP-1, Jip1, Prkm8ip, Skip, mjip-2a)
• Tag / Fusion Protein:
  • EE (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a few mismatches between the depositor's reference sequence and Addgene's quality control sequence. The changes are upstream of the JIP1 ORF and should not affect its function.