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Purpose(Empty Backbone) a bicistronic IRES-GFP containing vector for mammalian expression (CMV promoter)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52116 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCI-neo
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Backbone manufacturerPromega
- Backbone size (bp) 6765
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- IRES-EGFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIGneo was a gift from Dario Vignali (Addgene plasmid # 52116 ; http://n2t.net/addgene:52116 ; RRID:Addgene_52116)
