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Purpose(Empty Backbone) a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSCV IRES GFP
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Modifications to backboneexpanded MCS and switched FP
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Vector typeMammalian Expression, Retroviral
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Tag
/ Fusion Protein
- IRES-ECFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN_5_primer (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The FP can be removed with Nco1-Not1 digest.
The Multicloning site reads EcoR1-BglII-SnaB1-BamH1-Mfe1-Xho1. These are unique. The Nhe1 site is not unique.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-IRES-CFP II (pMIC II) was a gift from Dario Vignali (Addgene plasmid # 52109 ; http://n2t.net/addgene:52109 ; RRID:Addgene_52109) -
For your References section:
Generation of T-cell receptor retrogenic mice. Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA. Nat Protoc. 2006;1(1):406-17. 10.1038/nprot.2006.61 PubMed 17406263
