pMSCV-IRES-GFP II (pMIG II)
(Plasmid #52107)

• Purpose: (Empty Backbone) a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP
• Depositing Lab: Dario Vignali
• Publication: Holst et al Nat Protoc. 2006;1(1):406-17.

Backbone

• Vector backbone: MSCV IRES GFP
• Modifications to backbone: expanded MCS
• Vector type: Mammalian Expression, Retroviral
• Tag / Fusion Protein:
  • IRES-EGFP (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN_5_primer

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 38 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The FP can be removed with Nco1-Not1 digest.
The Multicloning site reads EcoR1-BglII-SnaB1-BamH1-Mfe1-Xho1. These are unique. The Nhe1 site is not unique.

Information for Cloning Grade DNA (Catalog # 52107-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here