cp196V1.2
(Plasmid
#52100)
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PurposeA improved cpmCherry variant
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52100 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD/HisB
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 4880
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namecp196V1.2
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Alt nameimproved circularly permuted mCherry variant
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SpeciesSynthetic
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Insert Size (bp)780
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GenBank IDKJ534665
- Promoter pBAD promotor
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Tag
/ Fusion Protein
- 6 His tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pBAD-Forward primer
- 3′ sequencing primer pBAD-Reverse primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cp196V1.2 was a gift from Robert Campbell (Addgene plasmid # 52100 ; http://n2t.net/addgene:52100 ; RRID:Addgene_52100) -
For your References section:
Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry. Carlson HJ, Campbell RE. Protein Eng Des Sel. 2013 Dec;26(12):763-72. doi: 10.1093/protein/gzt052. Epub 2013 Oct 22. 10.1093/protein/gzt052 PubMed 24151339