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Addgene

CH-GECO2.1
(Plasmid #52099)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52099 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/HisB
  • Backbone size w/o insert (bp) 4100
  • Total vector size (bp) 6388
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CH-GECO2.1
  • Alt name
    calcium indicator based on circular permutated mCherry
  • Species
    Synthetic; synthetic construct
  • Insert Size (bp)
    1266
  • GenBank ID
    KJ534664
  • Promoter pBAD promotor
  • Tag / Fusion Protein
    • 6 His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI before 6xHis, NheI after 6xHis (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBAD-Forward primer
  • 3′ sequencing primer pBAD-Reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CH-GECO2.1 was a gift from Robert Campbell (Addgene plasmid # 52099 ; http://n2t.net/addgene:52099 ; RRID:Addgene_52099)

  • For your References section:

    Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry. Carlson HJ, Campbell RE. Protein Eng Des Sel. 2013 Dec;26(12):763-72. doi: 10.1093/protein/gzt052. Epub 2013 Oct 22. 10.1093/protein/gzt052 PubMed 24151339
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