pLenti-EmGFP-LATS2/1 KD
(Plasmid #52085)

• Purpose: Lentiviral RNAi vector for knockdown of LATS2 and LATS1. Co-expresses EmGFP as reporter
• Depositing Lab: Yutaka Hata
• Publication: Yang et al Mol Cell Biol. 2014 Feb 18.

Backbone

• Vector backbone: pLenti4-EF/V5-DEST
• Modifications to backbone: EmGFP and LATS2/1 KD were inserted into pLenti-EF/V5-DEST from pcDNA 6.2-GW/miR vector.
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: EmGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LATS2 and LATS1 miRNAs
• Species: H. sapiens (human)
• Entrez Gene: LATS1 (a.k.a. WARTS, wts)
• Entrez Gene: LATS2 (a.k.a. KPM)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: pBABE-3

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Co-cistronic expression of LATS2/1 pre-miRNA with EmGFP as reporter.