pLenti-EF-FH-TAZ S89A-ires-blast
(Plasmid #52084)

• Purpose: Lentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tags
• Depositing Lab: Yutaka Hata
• Publication: Yang et al Mol Cell Biol. 2014 Feb 18.

Backbone

• Vector backbone: pLenti-EF-ires-blast
• Modifications to backbone: pLenti-EF-FH-TAZ S89A was constructed by subcloning NheI/SalI fragment from pCIneoFH-TAZ S89A into pLenti-EF-ires-blast vector. TAZ insert was amplified by PCR, cut with EcoRI and XhoI and ligated into EcoRI and Sall sites of pLenti-EF-ires-blast vector.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TAZ S89A
• Alt name: Transcriptional co-activator with PDZ-binding motif
• Alt name: WWTR1
• Species: H. sapiens (human)
• Mutation: S89A
• Entrez Gene: WWTR1 (a.k.a. TAZ)
• Promoter: EF1a
• Tags / Fusion Proteins:
  • FLAG (N terminal on backbone)
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: pBABE-3

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that TAZ has a Sall site present within the coding region.