PsuDJ-1.1
(Plasmid
#52080)
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PurposeEncodes Panagrolaimus superbus DJ-1. Potent antioxidant and molecular chaperone
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52080 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-30 EK/LIC
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Backbone manufacturerMerck Millipore
- Backbone size w/o insert (bp) 5439
- Total vector size (bp) 5997
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Growth instructionsfor plasmid propagation xl1-blue or top10 cells. Protein production BL21 DE3 pLysS.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePsuDJ-1.1
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SpeciesPanagrolaimus superbus
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Insert Size (bp)558
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GenBank IDKJ522972
- Promoter T7
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Tag
/ Fusion Protein
- His Tag (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PsuDJ-1.1 was a gift from Ann Burnell (Addgene plasmid # 52080 ; http://n2t.net/addgene:52080 ; RRID:Addgene_52080)