EcAR7
(Bacterial strain #52055)

• Purpose: Strain for use in Rinehart Lab Phosphoprotein synthesis kit
• Depositing Lab: Jesse Rinehart
• Publication: Heinemann et al FEBS Lett. 2012 Oct 19;586(20):3716-22. doi: 10.1016/j.febslet.2012.08.031. Epub 2012 Sep 13.

Backbone

• Vector backbone: None

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 30°C
• Growth Strain(s): The gene encoding phosphoserine phosphatase (serB) was mutated within the genome of rEc7 delta prfA delta tolC.bla:T7
• Growth instructions: Strain grows slowly.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Resource Information

• Supplemental Documents:
  • Users Guide for Phosphoprotein reagents.pdf
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We recommend growth of the EcAR7 strain at 30 °C in LB supplemented with 0.08% glucose. The EcAR7 strains normally take 1.5-­‐2 days to grow at 30°C, after transformation or streaking the strain on an agar plate (with the appropriate antibiotics).
See supplemental information from referenced article for protocol on creating EcAR7 from EcNR2.