pTet-O-Nd1-puro
(Plasmid #52052)

• Purpose: Expresses Nd1 and puromycin resistent gene under control of TetON promoter
• Depositing Lab: Marius Wernig
• Publication: Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10.1016/j.neuron.2013.05.029.

Backbone

• Vector backbone: Tet-O-FUW
• Backbone size w/o insert (bp): 7745
• Total vector size (bp): 10140
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Neurod1
• Species: M. musculus (mouse)
• Insert Size (bp): 1071
• GenBank ID: NM_010894.2
• Entrez Gene: Neurod1 (a.k.a. BETA2, BHF-1, Nd1, Neurod, bHLHa3)
• Promoter: tet-on
• Tag / Fusion Protein:
  • T2A-and puromycin resistant cassette (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcorR1 (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: WPRE-R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's quality control sequence shows that there is a minor change in the T2A region, EF to TR; Nd1 itself or the Puro cassette are not affected.