GFP-UBR5 ∆HECT
(Plasmid #52051)

• Purpose: CMV driven mammalian expression of ligase-dead UBR5 with an N-termianl GFP fusion
• Depositing Lab: Darren Saunders
• Publication: Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 10.1016/j.cell.2012.06.039. Epub 2012 Aug 9.

Backbone

• Vector backbone: pcDNA 6.2 N-EmGFP-DEST-V5
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5887
• Total vector size (bp): 14287
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Large plasmid. Can be difficult to produce high yield.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UBR5 ∆HECT
• Alt name: EDD
• Alt name: EDD1
• Alt name: hHYD
• Species: H. sapiens (human)
• Insert Size (bp): 8400
• Mutation: C2769A
• GenBank ID: NM_001282873.1
• Entrez Gene: UBR5 (a.k.a. DD5, EDD, EDD1, HYD)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: V5 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cDNA derived from pEGFP-C1 EDD (already submitted to addgene: https://www.addgene.org/37190/)
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid encodes a C-terminal V5 fusion, however this is not included as UBR5 cDNA insert contains 3' STOP codon.

A K503R mutation has been identified within the UBR5 translation. There is no information on the functional consequence of this mutation