pTet-O-Ngn2-puro
(Plasmid #52047)

• Purpose: 3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter
• Depositing Lab: Marius Wernig
• Publication: Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10.1016/j.neuron.2013.05.029.

Backbone

• Vector backbone: Tet-O-FUW
• Backbone size w/o insert (bp): 7745
• Total vector size (bp): 9861
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ngn2
• Species: M. musculus (mouse)
• Insert Size (bp): 1071
• GenBank ID: NM_009718.2
• Entrez Gene: Neurog2 (a.k.a. Atoh4, Math4A, bHLHa8, ngn-2, ngn2)
• Promoter: tet-on
• Tag / Fusion Protein:
  • T2A-and puromycin resistant cassette (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: WPRE-R

Resource Information

• Articles Citing this Plasmid:
  • 44 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry