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Addgene

pCR4-Dact1(1250-1601)
(Plasmid #52046)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52046 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR4-TOPO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dact1(1250-1601)
  • Alt name
    Dapper1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    352
  • Entrez Gene
    Dact1 (a.k.a. 4921528D17Rik, AI115603, DAPPER, DAPPER1, FRODO, Frd1, Frodo1, MDpr1, MTNG3, THYEX3)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer M13 Reverse Priming
  • 3′ sequencing primer M13 Forward Priming
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR4-Dact1(1250-1601) was a gift from Ben Cheyette (Addgene plasmid # 52046 ; http://n2t.net/addgene:52046 ; RRID:Addgene_52046)

  • For your References section:

    Dact1 presomitic mesoderm expression oscillates in phase with Axin2 in the somitogenesis clock of mice. Suriben R, Fisher DA, Cheyette BN. Dev Dyn. 2006 Nov;235(11):3177-83. 10.1002/dvdy.20968 PubMed 17013874
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