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Addgene

pDYH5
(Plasmid #52032)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD95.81
  • Backbone manufacturer
    Andrew Fire (Addgene Plasmid 1497)
  • Backbone size w/o insert (bp) 4406
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DAF-16
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    7672
  • Entrez Gene
    daf-16 (a.k.a. CELE_R13H8.1)
  • Tags / Fusion Proteins
    • HIS (C terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer M13-Rev
  • 3′ sequencing primer GFP-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert DNA a gift from Thomas E. Johnson (University of Colorado, Boulder)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The D->V point mutation in DAF-16 has no functional consequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDYH5 was a gift from Meng-Qiu Dong (Addgene plasmid # 52032 ; http://n2t.net/addgene:52032 ; RRID:Addgene_52032)

  • For your References section:

    CAMKII and Calcineurin regulate the lifespan of Caenorhabditis elegans through the FOXO transcription factor DAF-16. Tao L, Xie Q, Ding YH, Li ST, Peng S, Zhang YP, Tan D, Yuan Z, Dong MQ. Elife. 2013;2:e00518. doi: 10.7554/eLife.00518. Epub 2013 Jun 25. 10.7554/eLife.00518 PubMed 23805378
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