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PurposeExpresses HA-Daxx in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52023 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHA-Daxx
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Alt namedeath-domain associated protein
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SpeciesH. sapiens (human)
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Entrez GeneDAXX (a.k.a. BING2, DAP6, EAP1, SMIM40)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-Daxx/pRK5 was a gift from Xiaolu Yang (Addgene plasmid # 52023 ; http://n2t.net/addgene:52023 ; RRID:Addgene_52023) -
For your References section:
Phosphorylation of Daxx by ATM contributes to DNA damage-induced p53 activation. Tang J, Agrawal T, Cheng Q, Qu L, Brewer MD, Chen J, Yang X. PLoS One. 2013;8(2):e55813. doi: 10.1371/journal.pone.0055813. Epub 2013 Feb 6. 10.1371/journal.pone.0055813 PubMed 23405218
