pcDNA3-MAVS-M142A-invitro(E13)
(Plasmid
#52017)
-
PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation M142A
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52017 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMAVS-M142A
-
Mutationchanged Met 142 to Ala
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Has extra restriction sites upstream of MAVS start codon. HindIII and XhoI
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3-MAVS-M142A-invitro(E13) was a gift from Jonathan Kagan (Addgene plasmid # 52017 ; http://n2t.net/addgene:52017 ; RRID:Addgene_52017) -
For your References section:
A Bicistronic MAVS Transcript Highlights a Class of Truncated Variants in Antiviral Immunity. Brubaker SW, Gauthier AE, Mills EW, Ingolia NT, Kagan JC. Cell. 2014 Feb 13;156(4):800-11. doi: 10.1016/j.cell.2014.01.021. 10.1016/j.cell.2014.01.021 PubMed 24529381