pcDNA3-HA-MAVS-M142A(KaganF61)
(Plasmid
#51999)
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PurposeExpresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51999 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMAVS
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MutationMet 142 to Ala
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-HA-MAVS-M142A(KaganF61) was a gift from Jonathan Kagan (Addgene plasmid # 51999 ; http://n2t.net/addgene:51999 ; RRID:Addgene_51999) -
For your References section:
A Bicistronic MAVS Transcript Highlights a Class of Truncated Variants in Antiviral Immunity. Brubaker SW, Gauthier AE, Mills EW, Ingolia NT, Kagan JC. Cell. 2014 Feb 13;156(4):800-11. doi: 10.1016/j.cell.2014.01.021. 10.1016/j.cell.2014.01.021 PubMed 24529381