pUC119-SA12
(Plasmid
#51995)
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PurposeContains the complete genome of the SA12 polymavirus
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51995 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC119
- Backbone size w/o insert (bp) 3162
- Total vector size (bp) 8392
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Modifications to backboneSA12 was cloned into the unique PstI site of pUC119.
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSA12
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Alt nameSimian virus 12
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Alt nameSimian agent 12
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SpeciesSimian virus 12
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Insert Size (bp)5230
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GenBank IDAY614708.1
- Promoter none
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC119-SA12 was a gift from James Pipas (Addgene plasmid # 51995 ; http://n2t.net/addgene:51995 ; RRID:Addgene_51995) -
For your References section:
Complete nucleotide sequence of polyomavirus SA12. Cantalupo P, Doering A, Sullivan CS, Pal A, Peden KW, Lewis AM, Pipas JM. J Virol. 2005 Oct;79(20):13094-104. 10.1128/JVI.79.20.13094-13104.2005 PubMed 16189011