PA-TNXL/pcDNA3
(Plasmid
#51994)
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Purposemammalian expression of a 'caged' Ca2+ indicator for fluorescent imaging at specific locations and time points
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51994 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePA-TNXL
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SpeciesSynthetic
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Insert Size (bp)1900
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank accession number: AB773422
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PA-TNXL/pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 51994 ; http://n2t.net/addgene:51994 ; RRID:Addgene_51994) -
For your References section:
Highlighted Ca(2)(+) imaging with a genetically encoded 'caged' indicator. Matsuda T, Horikawa K, Saito K, Nagai T. Sci Rep. 2013;3:1398. doi: 10.1038/srep01398. 10.1038/srep01398 PubMed 23474844
Map uploaded by the depositor.
