Nano-lantern(ATP1)/pRSETB
(Plasmid #51988)

• Purpose: bacterial expression of a Nano-lantern-based luminescent ATP indicator
• Depositing Lab: Takeharu Nagai
• Publication: Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.

Backbone

• Vector backbone: pRSETB
• Backbone manufacturer: Invitrogen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nano-lantern_ATP1
• Species: Synthetic
• Insert Size (bp): 2000
• Mutation: S257G in Rluc8
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GenBank accession number: JN859502. Although they differ by only three N-terminal amino acidsin the RLuc8-S257G moiety, their dynamic range and brightness were vastly different. Nano-lantern (ATP1) exhibited enough dynamic range (200%) for functional imaging, and was 1.5-fold brighter than Nano-lantern (ATP0) at ATP-saturating condition. They had similar affinities for ATP.