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Addgene

Nano-lantern-plasma membrane/pcDNA3
(Plasmid #51975)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nano-lantern
  • Species
    Synthetic
  • Insert Size (bp)
    1600
  • Mutation
    S257G in Rluc8
  • Tags / Fusion Proteins
    • Lyn (N terminal on insert)
    • HIS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank accession number: JN859492

The minor discrepancies between the QC and depositor sequence have no functional consequence and Addgene's QC sequence should be used for comparison.

Please note the upstream KpnI site shown on the depositor's map is not present in this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nano-lantern-plasma membrane/pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 51975 ; http://n2t.net/addgene:51975 ; RRID:Addgene_51975)

  • For your References section:

    Luminescent proteins for high-speed single-cell and whole-body imaging. Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, Yoshida Y, Matsuda T, Arai Y, Nagai T. Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248. 10.1038/ncomms2248 PubMed 23232392
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