Nano-lantern-mitochondria/pcDNA3
(Plasmid
#51973)
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Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to mitochondria
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51973 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNano-lantern
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SpeciesSynthetic
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Insert Size (bp)1800
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MutationS257G in Rluc8
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Tag
/ Fusion Protein
- CoxVIIIx2 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank accession number: JN859492
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Nano-lantern-mitochondria/pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 51973 ; http://n2t.net/addgene:51973 ; RRID:Addgene_51973) -
For your References section:
Luminescent proteins for high-speed single-cell and whole-body imaging. Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, Yoshida Y, Matsuda T, Arai Y, Nagai T. Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248. 10.1038/ncomms2248 PubMed 23232392
Map uploaded by the depositor.
