Nano-lantern-H2B/pcDNA3
(Plasmid #51971)

• Purpose: mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B
• Depositing Lab: Takeharu Nagai
• Publication: Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nano-lantern
• Species: Synthetic
• Insert Size (bp): 2000
• Mutation: S257G in Rluc8
• Tag / Fusion Protein:
  • human H2B (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GenBank accession number: JN859492

The minor discrepancies between the QC and depositor sequence have no functional consequence.