Nano-lantern/pRSETB
(Plasmid #51969)

• Purpose: bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging
• Depositing Lab: Takeharu Nagai
• Publication: Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.

Backbone

• Vector backbone: pRSETB
• Backbone manufacturer: Invitrogen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nano-lantern
• Species: Synthetic
• Insert Size (bp): 1600
• Mutation: S257G in Rluc8
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GenBank accession number: JN859492. The improved brightness of Nano-lantern should generate power densities in the range of 1μW/cm2 (versus 0.1μW/cm2 for RLuc8) following transient overexpression in the micromolar range in human cells, and thus increase imaging potential.