Yellow Cameleon-Nano65/pcDNA3
(Plasmid
#51965)
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Purposemammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYellow Cameleon-Nano65
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SpeciesSynthetic
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Insert Size (bp)2000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank accession number: HM145948
The minor discrepancies between the QC and depositor sequence have no functional consequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Yellow Cameleon-Nano65/pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 51965 ; http://n2t.net/addgene:51965 ; RRID:Addgene_51965) -
For your References section:
Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano. Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T. Nat Methods. 2010 Sep;7(9):729-32. doi: 10.1038/nmeth.1488. Epub 2010 Aug 8. 10.1038/nmeth.1488 PubMed 20693999
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/63/66/981c61a8-9e40-11e3-826d-000c29055998.png.940x940_q85_autocrop.png)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/34/73/fce9a510-9e4e-11e3-94e4-000c298a5150.png.940x940_q85_autocrop.png)