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Addgene

Ateam1.03-nD/nA/pRSETB
(Plasmid #51960)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51960 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSETB
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATeam1.03-nD/nA
  • Species
    Synthetic
  • Insert Size (bp)
    1800
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ateam1.03-nD/nA/pRSETB was a gift from Takeharu Nagai (Addgene plasmid # 51960 ; http://n2t.net/addgene:51960 ; RRID:Addgene_51960)

  • For your References section:

    Reversible dimerization of Aequorea victoria fluorescent proteins increases the dynamic range of FRET-based indicators. Kotera I, Iwasaki T, Imamura H, Noji H, Nagai T. ACS Chem Biol. 2010 Feb 19;5(2):215-22. doi: 10.1021/cb900263z. 10.1021/cb900263z PubMed 20047338
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