Skip to main content
Addgene

Phamret/pcDNA3
(Plasmid #51953)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51953 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phamret
  • Species
    Synthetic
  • Insert Size (bp)
    1400

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank accession number: AB435575. The cDNA of Phamret was constructed as a chimeric protein of C-terminally deleted mseCFP and N-terminally deleted PA-GFP. PA-GFP was constructed from wild-type GFP by substituting Valine at 163 for Alanine, Serine at 175 for Glysine, and Threonine at 203 for Histidine.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Phamret/pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 51953 ; http://n2t.net/addgene:51953 ; RRID:Addgene_51953)

  • For your References section:

    Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein. Matsuda T, Miyawaki A, Nagai T. Nat Methods. 2008 Apr;5(4):339-45. doi: 10.1038/nmeth.1193. Epub 2008 Mar 16. 10.1038/nmeth.1193 PubMed 18345008
Related items:
Commonly requested with: