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Addgene

mSECFP/pcDNA3
(Plasmid #51951)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51951 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mSECFP
  • Species
    Synthetic
  • Insert Size (bp)
    700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank accession number: AB435576. ECFP was modified to improve its brightness and oligomeric property by substituting Serine at 72 for Alanine, Serine at 175 for Glysine, and Alanine at 206 for Lysine, yielding mseCFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mSECFP/pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 51951 ; http://n2t.net/addgene:51951 ; RRID:Addgene_51951)

  • For your References section:

    Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein. Matsuda T, Miyawaki A, Nagai T. Nat Methods. 2008 Apr;5(4):339-45. doi: 10.1038/nmeth.1193. Epub 2008 Mar 16. 10.1038/nmeth.1193 PubMed 18345008
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