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Addgene

pRK24
(Bacterial strain #51950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 51950 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    RK24
  • Total vector size (bp) 60099

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ECNR2.ΔtolC.mutS:zeo [pRK24]
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    E. coli Conjugation genes
  • Promoter na

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LB Lennox with Tetracycline to maintain plasmid, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK24 was a gift from Farren Isaacs (Addgene plasmid # 51950)

  • For your References section:

    Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering. Ma NJ, Moonan DW, Isaacs FJ. Nat Protoc. 2014 Oct;9(10):2285-300. doi: 10.1038/nprot.2014.081. Epub 2014 Sep 4. 10.1038/nprot.2014.081 PubMed 25188631
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