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Addgene

pGL3-alphaMHC 3300bp
(Plasmid #51934)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51934 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Basic
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    alpha MHC
  • Alt name
    alpha mysosin heavy chain
  • Species
    R. norvegicus (rat)
  • Mutation
    -2930 to +410 promoter region
  • Entrez Gene
    Myh6 (a.k.a. Myhca)
  • Promoter alpha MHC
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-alphaMHC 3300bp was a gift from Jeffery Molkentin (Addgene plasmid # 51934 ; http://n2t.net/addgene:51934 ; RRID:Addgene_51934)
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