Alpha MHC-DN.JNK2
(Plasmid #51931)

• Purpose: mammalian expression of dominant negative JNK2
• Depositing Lab: Jeffery Molkentin
• Publication: Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Backbone

• Vector backbone: alphaMHC vector
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DN JNK2
• Alt name: JNK2
• Alt name: mitogen-activated protein kinase 9
• Alt name: MAPK9
• Species: M. musculus (mouse)
• Mutation: dominant negative: the dual phosphorylation motif TPY was changed to APF
• Entrez Gene: Mapk9 (a.k.a. JNK2, Prkm9, p54aSAPK)
• Promoter: cardiac-specific α-myosin heavy chain 5.5 kb promoter
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing found the following additional mutations: S2G, S6C and G8S, they not thought to effect the function of the insert.