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Addgene

pcDNA1-GATA6
(Plasmid #51929)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51929 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GATA6
  • Alt name
    GATA binding protein 6
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Gata6 (a.k.a. AA410133, GATA-6)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SalI site is 120bp 5' of ATG and XbaI site comes from bluescript.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA1-GATA6 was a gift from Jeffery Molkentin (Addgene plasmid # 51929 ; http://n2t.net/addgene:51929 ; RRID:Addgene_51929)

  • For your References section:

    The transcription factors GATA4 and GATA6 regulate cardiomyocyte hypertrophy in vitro and in vivo. Liang Q, De Windt LJ, Witt SA, Kimball TR, Markham BE, Molkentin JD. J Biol Chem. 2001 Aug 10;276(32):30245-53. Epub 2001 May 16. 10.1074/jbc.M102174200 PubMed 11356841
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