GP1BB
(Plasmid #51908)

• Purpose: Expresses full-length Platelet glycoprotein Ib beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
• Depositing Lab: Gavin Wright
• Publication: Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6618
• Total vector size (bp): 7085
• Modifications to backbone: Stop codon at amino acid 149, no translation of rat CD4 d3+4, enzymatic biotinylation sequence, linker and His tag (CD4d3+4-BioLHis).
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GP1BB
• Alt name: Platelet glycoprotein Ib beta chain precursor
• Species: H. sapiens (human)
• Insert Size (bp): 467
• Mutation: Stop Codon at amino acid 149, before CD4, bio and His tags
• Entrez Gene: GP1BB (a.k.a. BDPLT1, BS, CD42C, GPIBB, GPIbbeta)
• Promoter: CMV
• Tags / Fusion Proteins:
  • rat CD4 d3+4 (C terminal on backbone)
  • enzymatic biotinylation sequence (C terminal on backbone)
  • His tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV-fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert was chemically synthesised.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Article ref 77

Co-transfect with GP1BA-bio-His (Addgene 51907) to create the GP1BA/GP1BB dimer