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Addgene

pfast bac cry1myc
(Plasmid #51892)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51892 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pfastbac
  • Backbone manufacturer
    Invitrogen
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cry1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cry1 (a.k.a. Phll1)
  • Promoter polyhedrin promoter
  • Tags / Fusion Proteins
    • myc (C terminal on insert)
    • 3x Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer Polyhedrin forward
  • 3′ sequencing primer SV40pA-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pfast bac cry1myc was a gift from Joseph Takahashi (Addgene plasmid # 51892 ; http://n2t.net/addgene:51892 ; RRID:Addgene_51892)

  • For your References section:

    Competing E3 ubiquitin ligases govern circadian periodicity by degradation of CRY in nucleus and cytoplasm. Yoo SH, Mohawk JA, Siepka SM, Shan Y, Huh SK, Hong HK, Kornblum I, Kumar V, Koike N, Xu M, Nussbaum J, Liu X, Chen Z, Chen ZJ, Green CB, Takahashi JS. Cell. 2013 Feb 28;152(5):1091-105. doi: 10.1016/j.cell.2013.01.055. 10.1016/j.cell.2013.01.055 PubMed 23452855
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